Resealed erythrocyte act on acute lymphoblastic leukaemia, cancer of. Institute of pharmaceutical sciences, udaipur rajasthan 301 erythrocytes, also known as red blood cells, have been extensively studied for their potential carrier capabilities for the delivery of drugs and drugloaded microspheres. The use of erythrocytes as a controlled release of drug is predominantly devoted to small molecules that are minimally retained. Resealed erythrocytes are the promising drug carriers that can be used for targeted drug delivery systems minimizing the side effects of the conventional dosage form. Resealed erythrocytes, drug carriers, drug loading, drug efficiency introduction resealed erythrocytes are most commonly known drug delivery system. Blood or packed red blood cells were obtained from the university of michigan hospital blood bank.
Autologous red blood cell delivery of betamethasone phosphate. Design and charecterization of anticancer engineered resealed. A cell that contains hemoglobin and can carry oxygen to the body. Such drugloaded carrier erythrocytes are prepared simply by collecting blood samples from the organism of interest, separating erythrocytes from plasma, entrapping drug in the. Prior to use, 200 l of packed erythrocytes were washed 3x in 1 ml pbs or complete rpmi with albumax, and finally resuspended in 1 ml pbsmedium for use. Camag software cats was used for peak height and peak. Applications of resealed erythrocytes erythrocytes as carrier for enzymes erythrocytes as carrier for drugs erythrocytes for drug targeting drug targeting to reticuloendothelial system drug. The components of the degraded erythrocytes hemoglobin are further processed as follows. Pdf preparation and in vitro evaluation of resealed erythrocytes. Resealed erythrocytes a specified tool in novel drug.
Formulation and drug loading features of nanoerythrocytes. The use of cells as drug delivery system requires that the drug which are normally unable to permeate the membrane, should be made to traverse the membrane without causing any irreversible changes in membrane structure and. Jan, 2014 contents of the powerpoint on resealed erythrocytes include. The use of resealed erythrocytes shows potential for a safe and effective delivery of various bioactive molecules for effective targeting. Such erythrocytes can act as carriers that prolong the drugs action due to its. Resealed erythrocytes, 5flurouracil, encapsulation, hypotonic preswelling, isotonicresealing. The main advantage of using resealed erythrocytes as drug targeting carrier. This book, the use of resealed erythrocytes, is based on the fourth meeting of the international society for the use of resealed erythrocytes as carriers and bioreactors i. Resealed erythrocytes serve as an ideal carrier for antineoplastic agents, antimicrobial drug and vitamins and steroids. Erythrocytes are the most abundant cells in the human body 5.
Institute of pharmaceutical sciences, udaipur rajasthan 301 erythrocytes, also known as red. The overall process is based on the response of these cells under osmotic. Resealed erythrocytes isolation of erythrocytes, drug loading in erythrocytes, factors effecting resealed erythrocytes m. Erythrocytes have been proposed as a carriers for a wide range of bioactive components including drugs enzymes, pesticides, dna molecules and others. Design and evaluation of drug loaded erythrocytes international. However, the normal range of rbcs count in adults is 700,000 to 5. Indeed, immediately after the stroke he showed increased values for hemoglobin, hematocrit and erythrocytes, in comparison with those observed at discharge one week before. Blood contains various type of cells like erythrocytes rbc,wbc and platelets, among them the rbc are the most interesting carrier and posses great. Contents of the powerpoint on self emulsifying drug delivery systems sedds include. Such cells could be used as circulating carriers to disseminate a drug within a. Wornout erythrocytes are removed from circulation and destroyed in the res of spleen and liver ritesh kumar et al. Formulation, characterization and invitro evaluation of. College of pharmacy, teerthanker mahaveer university, moradabad, uttar pradesh, india. Various advantages of resealed erythrocytes they are the natural product of the body, which are biodegradable in nature.
The study involved the usage of cross linking agents like dmf, dmso and glutaraldehyde for the formulation of paclitaxel resealed erythrocytes. Program for fundamental research fundamentals of the technology of. International journal of chemistry and pharmaceutical sciences 169 and serves only to carry haemoglobin. Data are presented which show that these resealed ghosts possess many of the permeability characteris tics of their parent native erythrocytes. Erythrocytes are biocompatible, biodegradable, possess long circulation half lives and can be loaded with a variety of biologica. Mar 17, 2017 nano erythrocyte ghosts have recently been used as drug carriers of watersoluble apis due to inherit biological characteristics of good compatibility, low toxicity, and small sideeffect. Erythrocytes constitute potential biocompatible vectors for different bioactive substances, including drugs, enzymes and proteins. But a foggy or hazy doublepane window doesnt necessarily need replacing. The resealed erythrocyte ghosts were washed three times and were. Fresh whole blood is the blood that is collected heparinized tubes by venipuncture withdrawn from cardiacsplenic puncture in a syringe containing a drop of. Formulation, characterization and invitro evaluation of resealed erythrocytes as a carrierfor antineoplastic agents article in international journal of pharma and bio sciences 54. The pores created by this method are resealed by using 154 mm. Hexaphosphate loaded red blood cells in various preparation conditions.
Various bioactive agents can be encapsulated to develop a sustained release formulation. Influence of the glycocalyx and plasma membrane composition. Such cells could be used as circulating carriers to disseminate a drug within a prolonged period of time in circulation or in targetspecific organs, including the liver, spleen, and lymph nodes. Resealed erythrocytes as drug carriers and its therapeutic applications. Erythrocytes are produced in the bone marrow and sent into the circulation. This book, the use of resealed erythrocytes, is based on the fourth meeting of. Red blood cells erythrocytes the red blood cells are highly specialized, well adapted for their primary function of transporting oxygen from the lungs to all of the body tissues. The drug is entrapped in the erythrocytes, and resealing the resultant cellular carriers. Erythrocytes from all mammals are anucleated, and most are in the shape of biconcave discs called discocytes figs. Applications of resealed erythrocytes resealed erythrocytes have several possible applications in various fields of human and veterinary medicine. In this communication, we describe the preparation and characterization of hormonesensitive resealed ghosts from turkey erythrocytes. Until other carrier systems come of age, resealed erythrocytes technology will remain an active field for the further research. Resealed erythrocytes are prepared by isolating erythrocytes from the plasma of the blood sample of an organism of interest, breakdown of cells using different methods and then entrapping the drug which is to be administered into the body into the erythrocytes by various techniques such as. Erythrocytes live up to 120 days in the circulation, after which the wornout cells are removed by a type of myeloid phagocytic cell called a macrophage, located primarily within the bone marrow, liver, and spleen.
May 26, 2015 by using various methods the cells are broken and the drug is entrapped into the erythrocytes, finally they are resealed and the resultant carriers are then called resealed erythrocytes. Franklin lakes, nj, usa and with its accompanying software cellquest. First of all, we would like to welcome all of you to this new website. Applications of resealed erythrocytes erythrocytes as carrier for enzymes erythrocytes as carrier for drugs erythrocytes for drug targeting drug targeting to reticuloendothelial system drug targeting to liver treatment of liver tumors treatment of parasitic diseases removal of res iron overload removal of toxic agents 21. Various bioactive agents encapsulated in erythrocytes are developed for the slow and sustained release in circulation to allow effective. The entrapment of drug does not require the chemical modification of the substance. Resealed erythrocytes a specified tool in nov a specified tool in. Nano erythrocyte ghosts have recently been used as drug carriers of watersoluble apis due to inherit biological characteristics of good compatibility, low toxicity, and small sideeffect. Preparation and characterization of hormonesensitive. Preparation and in vitro evaluation of resealed erythrocytes as a new trend in treatment of asthma. Formulation and drug loading features of nano erythrocytes xiaoting dong1, yawei niu1, yi ding2, yuemin wang1, jialan zhao1, wei leng3 and linghao qin1 abstract nano erythrocyte ghosts have recently been used as drug carriers of watersoluble apis due to inherit biological characteristics of good compatibility, low toxicity, and small sideeffect.
The present investigation reports the formulation of resealed erythrocytes rbcs as carriers for the anticancer agent, 5fluorouracil 5fu. At the end of their lifecycle, they are destroyed by macrophages, and their components are recycled. Formulation and drug loading features of nanoerythrocytes xiaoting dong1, yawei niu1, yi ding2, yuemin wang1, jialan zhao1, wei leng3 and linghao qin1 abstract nano erythrocyte ghosts have. In this pharma innovative world, there are more than 30 drug delivery systems. Contents of the powerpoint on resealed erythrocytes include. Various bioactive agents encapsulated in erythrocytes are developed for the slow and sustained release in circulation to allow effective treatment of parasitic diseases. Isolation of erythrocytes is easy and larger amount of drug can be encapsulated in a small volume of cells. Such cells could be used as circulating carriers to. Use of resealed erythrocytes as delivery system for c. Effective drug targeting by erythrocytes as carrier systems. Resealed erythrocytes a specified tool in novel drug delivery.
Design and charecterization of anticancer engineered. Numerous application have been proposed for the use of resealed erythrocytes as carrier for drugs, enzyme replacement therapy etc. Formulation and drug loading features of nanoerythrocytes ncbi. Erythrocytes from mammalians like mice, cattle, pigs, dogs, sheep, goats, monkeys, chicken, rats and rabbits. Oct 02, 20 applications of resealed erythrocytes erythrocytes as carrier for enzymes erythrocytes as carrier for drugs erythrocytes for drug targeting drug targeting to reticuloendothelial system drug targeting to liver treatment of liver tumors treatment of parasitic diseases removal of res iron overload removal of toxic agents 21. Erythrocytes also known as red blood cells and have extensively studied for their potential carrier capabilities for delivery of drugs. Resealed erythrocytes as drug carriers and its therapeutic.
Resealed erythrocytes as a carrier for drug targeting. The definition of high or low blood cell count is relative depending on medical practice. Resealed erythrocytes fundamentals and applications, formulation, preparation methods and evaluation. Resealed erythrocytes as drug carriers pharmatutor. International journal of chemistry and pharmaceutical sciences. Erythrocytes, the most abundant cells in the human body, have potential carrier capabilities for the delivery of drugs. Isolation of erythrocytes is easy and larger amount of drug can be.
Bsp was loaded into rat autologous erythrocytes by hypotonic preswelling method. Such drugloaded carrier erythrocytes are prepared simply by collecting blood samples from the organism of interest, separating erythrocytes from plasma, entrapping drug in the erythrocytes, and resealing the resultant cellular carriers. Jul 14, 2015 human erythrocytes were stored in packed volume at 4c. Hence, these carriers are called resealed erythrocytes.
Resealed erythrocytes have several possible applications in various fields of human and veterinary medicine. Swathi paskanti 1assistant professor, 2,3,4student of b. Resealed erythrocytes, 5flurouracil, encapsulation, hypotonicpreswelling, isotonicresealing. Resealed erythrocytes are gaining more popularity because of their biocompatibility, biodegradability, ability to circulate throughout the body, ease of preparation and ability to perform extended release. The clear supernatant is analyzed for the drug content by spectroscopy method. Design and characterization of anticancer engineered resealed erythrocytes 245 osmolality 0 300 mosmkg at room temperature over 30 min.
The drug is entrapped in the erythrocytes, and resealing the. It contains information about erythrocytes red blood cells in general, their rheological behavior and the. College of pharmacy, teerthanker mahaveer university, moradabad, uttar pradesh, india erythrocytes have been the most interesting carrier and have found to possess great potential in drug targeting. Preparation and in vitro evaluation of resealed erythrocytes as a new trend in treatment of asthma mohammed f ibrahim, alaa zaky, mohsen i afouna, ahmed m samy department of pharmaceutics and industrial pharmacy, college of pharmacy boys, alazhar university, nasr city, cairo, egypt. The in vitro release of methotrexate from the resealed erythrocytes formulation was determined using dialysis cassettes slidealyzer3. In this study, we developed a novel drug delivery system based on nano erythrocyte ghosts stsnanorbcs to transport sodium tanshinone iia sulfonate sts for intravenous use in rat. Therefore, it is parental control release formulation. Introduction definition and advantages formulation of ppt resealed erythrocytes. Hence, these riers are called resealed erythrocytes. The size, shape, and number of erythrocytes, and the number of hemoglobin molecules can have a major impact on a persons health. And because of this resealing process these carriers are called resealed erythrocytes. Erythrocytes as carrier for drugs, enzymes and peptides. Pharm sonam gandhi presentation slides pharmacology publish your. Resealed erythrocytes a specified tool in novel drug delivery system vaishali goel, charanjeet kaur, rainu devi, aman kumar, ujjwal nautiyal himachal institute of pharmacy, paonta sahib, distt.
The use of resealed erythrocytes as carriers and bioreactors. Resealed erythrocytes are used as a carrier to target res and nonres systems. Surfacemodified erythrocytes are used to target organs of mononuclear phagocytic system reticuloendothelial system because the changes in the membrane are recognized by macrophages. Feb 11, 2016 drugloaded erythrocytes as a slowrelease system. The overall process is based on the response of these cells under osmotic conditions. This blood is stored in cpda 1 citrate, phosphate, dextrose and adenine and has an expiration date 35 days after drawing. The use of erythrocytes as a controlled release of drug is predominantly devoted to small molecules that are minimally retained within erythrocytes and progressively leak from the cell into the plasmatic compartment. The commercial software dp soft is used for controlling the camera, taking the. Lets face it, sometimes windows are a pane in the glass. Erythrocytes red blood cells or rbcs are anucleate, biconcave cells, filled with hemoglobin, that transport oxygen and carbon dioxide between the lungs and tissues.
In vivo evaluation of drugloaded erythrocytes tissue distribution studies in vivo studies were carried out in albino rats weighing 250 20 gm to find out the survival time of 5fu loaded erythrocytes. Resealed erythrocytes as a carrier meenu sharma, saroj jain department of pharmaceutics, hindu college of pharmacy panchayat bhawan, gohana road, sonipat, haryana, india. Resealed erythrocyte free download as powerpoint presentation. By using various methods the cells are broken and the drug is entrapped into the erythrocytes, finally they are resealed and the resultant carriers are then called resealed erythrocytes. Potential carrier for the delivery of drugs and drugloaded microspheres 1ms. Data are presented which show that these resealed ghosts possess.
The steps involved in the preparation of drugloaded carrier erythrocytes include. Use of resealed erythrocytes as delivery system for creactive protein crp to generate macrophagemediated tumoricidal activity. Upon reinjection, the drugloaded erythrocytes serve as slow circulating depots and target the drugs to a reticuloendothelial system res. Introduction erythrocytes, the most abundant cells in the human. Entrapment by endocytosis endocytosis involves the addition of one volume of washed packed erythrocytes to nine volumes of buffer containing 2. Preparation and in vitro evaluation of resealed erythrocytes. Such drugloaded carrier erythrocytes are prepared simply by collecting blood samples from the organism of interest, separating erythrocytes from plasma, entrapping drug in the erythrocytes, and resealing the resultant cellular carriers 6. Briefly, 4 ml of erythrocytes suspension containing known amount of drug was placed in a dialysis cassettes previously soaked in distilled water for 24 hrs. To prepare experimental samples, 24 l of this cell stock 48 million cells were used per sample. Surfacemodified erythrocytes are used to target organs of mononuclear phagocytic system reticuloendothelial system because the. Resealed erythrocytes are prepared by collecting blood from the interested organism, separating erythrocytes from plasma, trapping the drug in erythrocytes, and resealing the resultant cellular. Resealed erythrocytes can act as drug carriers and targeting tools as well.